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Partial biochemical maturation of aneurally cultured human skeletal muscle

Department of Neurology, University of Cincinnati Medical Center (Drs. Samaha and Nagy), and the Division of Pediatric Neurology, Children's Hospital Medical Center (Dr. lannaccone), Cincinnati, OH.

We studied cultures of human skeletal muscle in vitro and estabilshed standards for biochemical markers of cellular differentiation. DNA synthesis ceased at the time of fusion, implying the absence of fibroblasts. Myosin heavy-chain synthesis, creatine and pyruvate kinase activities, and isoenzymes of creatine kinase were measured serially over 36 days. Filamin and fibronectin proteins were identified in these cultures. Compared to chick muscle in culture, human skeletal muscle cells remained relatively immature. These data provide a basis for the study of diseased human muscle cells in culture.

Address correspondence and reprint requests to Dr. Samaha, Department of Neurology, University of Cincinnati Medical Center, 231 Bethesda Avenue, Cincinnati, OH 45267.

This work was presented in part at the thirty-third annual meeting of the American Academy of Neurology, Toronto, Canada, April 1981.

This work was supported by a grant from the Muscular Dystrophy Association.

Accepted for publication December 15, 1981.

This article has been cited by other articles:

				S. T. Iannaccone, B. Nagy, and F. J. Samaha Decreased Creatine Kinase Activity in Cultured Duchenne Dystrophic Muscle Cells J Child Neurol, January 1, 1987; 2(1): 17 - 21. [Abstract] [PDF]