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Duchenne muscular dystrophy

Adenosine triphosphate and creatine phosphate content in muscle

Department of Neurology, University of Cincinnati Medical Center, Cincinati, OH.

In carrying out a new study of nucleotide concentrations in dystrophic muscle, we utilized myosin as a reference base. In nine control vastus lateralis muscle samples, nucleotide concentrations were 105 211 (SEM) moles of adenosine triphosphate (ATP) and 446 ± 57 (SEM) moles of creatine phosphate (CP) per milligram of myosin. In seven Duchenne dystrophic vastus lateralis muscle samples, nucleotide concentrations were 127 ± 25 (SEMI nmoles of ATP and 462 ± 119 (SEM) moles of CP per milligram of myosin. The CPATP ratio of 3.6 2 0.367 from Duchenne muscle was not signiificantly different from normal, 4.32 ± 0.243 ((p <0.20). In addition, myokinase activity, which is automatically assayed in these assays, was significantly increased in the Duchenne samples (p <0.10). The percent of myosin per total protein in the Duchenne biopsies was also diminished by 29% (p <0.01). These studies suggest that ATP and CP concentrations are not decreased in Duchenne dystrophic muscle when expressed in relation to the amount of contractile protein.

Address correspondence and reprint requests to Dr. Samaha, Department of Neurology, University of Cincinnati Medical Center, MSB 4010, Cincinnati, OH 45267.

Supported by the Muscular Dystrophy Association.

Presented in part at the thirty-second annual meeting of the American Academy of Neurology, New Orleans, LA, April 1980.

Accepted for publication September 3, 1980.

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