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Histochemical labeling of ß-adrenergic receptors in the mouse central nervous system by 9-amino-acridin propranolol

Department of Neurology, Hadassah University Hospital and Hebrew University-Hadassah Medical School, Jerusalem, and the Department of Biological Chemistry, Hebrew University. Jerusalem, Israel.

A new fluorescent ß-adrenergic antagonist, 9-amino-acridin propranolol (9-AAP), was administered intravenously to living mice. In the cerebral cortex, the highest concentration of 9-AAP was noted in the hippocampal formation, where it distinctly labeled the hippocampal pyramidal cell layer and the granule cell layer of the dentate gyrus. High uptake occurred in the pyramidal cell layer of the piriform cortex. In the neocortex, fluorescence was less dense and more diffuse but confined to the basal layers. A similar pattern was observed in the basal layers of the cingulate cortex, but an additional high-density dotted fluorescence labeled its layer II. In the cerebellar cortex, 9-AAP was localized within the Purkinje cell layer. In the spinal cord, the highest density of fluorescence was observed in the nuclear collections of -motoneurons. The findings were similar to those observed in the central nervous system of the rat and support the reproducibility of the method. 9-AAP may be used in vivo as a fluorescent probe to map out the central ß-adrenergic receptor system.

Dr. Melamed's address is Department of Neurology, Hadassah University Hospital and Hebrew University-Hadassah Medical School, Jerusalem, Israel.

Accepted for publication dune 20, 1977.